This survey was implemented to isolate, identify and analyze the susceptibility of HA-MRSA against commonly applied antibiotics as well as to detect its prevalence. Total 1394 samples of various natures were collected from different wards of Government Lady Reading Hospital Peshawar (Khyber Pakhtunkhwa) Pakistan. Staphylococcus aureus was isolated and identified by using selective media and biochemical tests including catalase, and coagulase tests, followed by detection of MRSA against methicillin. Further, susceptibility of MRSA was investigated against 14 antibiotics using standard disc-diffusion protocol. Demographic analyses revealed that out of 1394 samples, pus were the highest-rated (540) samples followed by urine (381) and blood (354) while the lowest number were semen samples (7). From total number of samples, highest incidences of Staphylococcus aureus were detected in pus (71.48%), blood (68.08%) and urine (54.35%) while lowest count was 42.86% in semen samples. Among Staphylococcus aureus-positive samples, MRSA rated 100%, 66.67%, 62.95%, 44.44%, 29.68%, 21.16%, 20.93% and 0.00% in semen, sputum, pus, throat-swab, urine, blood HVS and stools samples, respectively. All MRSA was found to possess average resistance of 64.08% (while 35.92% susceptibility) against all antibiotics collectively. Individually, highly resistant MRSA were recorded 98.71% against gentamicin followed by norfloxacin (82.14%) and ciprofloxacin (79.34%) while mostly susceptible to vancomycin (92.09%), chloramphenicol (67.09%) and sparfloxacin (57.40%). Overall, most adult female were found infected than male and children and vancomycin showed highest bactericidal property.
