Genetic Diversity of Olive Germplasm Using RAPD Markers

Abstract

Ten olive (Olea europaea L.) genotypes were collected from Agriculture Research Institute Tarnab at Peshawar and Sang Bhatti at Sawabi, KPK, Pakistan to evaluate their genetic diversity. Genomic DNA was isolated from fresh leaves, using a CTAB extraction method with few modifications. Three RAPD primers OPC-01, OPC-09 and OPE-07 obtained from Biron Technologies were tested by RAPD-PCR. Totally 27 bands were amplified and these amplified fragments ranged in size from 100 to 400 bp. Comparatively, more bands were observed in OPC-09 primer, with 12 bands and least number of bands were observed in OPC-01 primer with 7 bands from the electrophoresis results of the olive genotypes. Among three RAPD primers, only OPC-09 was polymorphic, showing (42%) polymorphism.